GRM 2010 GRM 2011

Abstract Details

Family Name:
First Name:
Title of Paper:
Environmental and genetic influences on chronic metabolic diseases What We Must Do?
Paper Proposal Text :
Environmental and genetic influences on chronic metabolic diseases What We Must Do?
Ghedeir Alshammari and Lomax M.
School of Bioscience, University of Nottingham, LE12 5RD, United Kingdom
Introduction: About 70% of people in the Gulf countries are obese and overweight. Obesity is a crucial health issue, being a risk factor for the development of chronic metabolic diseases such as type 2 diabetes, hypertension, hyperlipidemia, cardiovascular diseases, and some types of cancer. According to WHO, about 60% of deaths in the Gulf countries are caused by chronic metabolic diseases. Economic studies estimate that total health-care spending in these countries will reach $60 billion in 2025, up from $12 billion today and that the demand for hospital beds will more than double, requiring almost 162,000 beds to meet the explosion in the number of people suffering metabolic disease. Traditional methods to control obesity and overweight, by decreasing food intake, increasing exercise, and medical interventions have not been successful and more fundamental knowledge at the level of genes is required. It is known that the differences in fat distribution between subcutaneous and visceral depots in different people play an important role in the risk of developing chronic metabolic diseases but the causal mechanisms during development and role of genes have not been identified. The types of fat in our bodies can be increases or decreased by environmental factors, for instance cold weather stimulate some type of fat cells to burn energy more than to store it. It has been proposed that activation of genes involved in burning energy in these cells offers a future treatment for obesity and therefore proventing chronic metabolic diseases. However, most of health strategies in the Gulf countries are concentrating on social factors and unhealthy diets. Consequently, studies on the impact of genes in chronic metabolic diseases need to be supported by health strategies.
Hypothesis: We are examining the hypothesis that fat cells proliferation and differentiation can be altered by the impact of sex steroid hormones on genes regulating fat cell metabolism. Studies are in progress examining the effects of sex hormones on adipocyte proliferation and differentiation in white adipose cell lines and human fat cells prepared from male and females, normal and obese subjects from.
Method: Method:
1- Cell Culture:
White fat cells were grown in DMEM supplemented with 10% FBS.
2- Oil Red O staining:
Lipid accumulation in 3T3-L1 adipocytes was assessed by staining for 1hr in a freshly diluted Oil Red O.
3- Gene expression:
RNA was extracted, cDNA synthesised and mRNA quantified using standard qRTPCR.

Future work:
We are going to investigate whether fat genes in human cells can be affected by different ratios of physiological levels of sex steroids.